A mouse model for poliovirus neurovirulence identifies mutations that attenuate the virus for humans
N La Monica, JW Almond, VR Racaniello - Journal of virology, 1987 - Am Soc Microbiol
N La Monica, JW Almond, VR Racaniello
Journal of virology, 1987•Am Soc MicrobiolA mutation in the genome of poliovirus type 3 that is known to reduce neurovirulence in
humans similarly reduces neurovirulence in mice when incorporated into a mouse-adapted-
human poliovirus recombinant. Viral recombinants with a uracil at nucleotide position 472 in
the 5'-noncoding regions of their genomes are unable to replicate in the mouse brain. Viral
recombinants with a cytosine at this position are neurovirulent in mice. Neurovirulence of
poliovirus in mice may therefore prove to be a useful indicator of the genetic stability of new …
humans similarly reduces neurovirulence in mice when incorporated into a mouse-adapted-
human poliovirus recombinant. Viral recombinants with a uracil at nucleotide position 472 in
the 5'-noncoding regions of their genomes are unable to replicate in the mouse brain. Viral
recombinants with a cytosine at this position are neurovirulent in mice. Neurovirulence of
poliovirus in mice may therefore prove to be a useful indicator of the genetic stability of new …
A mutation in the genome of poliovirus type 3 that is known to reduce neurovirulence in humans similarly reduces neurovirulence in mice when incorporated into a mouse-adapted-human poliovirus recombinant. Viral recombinants with a uracil at nucleotide position 472 in the 5'-noncoding regions of their genomes are unable to replicate in the mouse brain. Viral recombinants with a cytosine at this position are neurovirulent in mice. Neurovirulence of poliovirus in mice may therefore prove to be a useful indicator of the genetic stability of new attenuating mutations created by site-directed mutagenesis.
American Society for Microbiology