The essential role of hypusine in eukaryotic translation initiation factor 4D (eIF-4D): purification of eIF-4D and its precursors and comparison of their activities
MH Park - Journal of Biological Chemistry, 1989 - ASBMB
Eukaryotic translation initiation factor 4D (eIF-4D) is the only protein known to contain the
amino acid, hypusine [N′-(4-amino-2-hydroxybutyl) lysine]. This unusual amino acid is
formed post-translationally by modification of a single specific lysine residue in an eIF-4D
precursor protein. Two separate eIF-4D precursors, each of which contains a lysine residue
in place of the hypusine residue and each of which thereby serves as a protein substrate for
the hypusine modification, were purified from DL-2-difluoromethylornithine-treated Chinese …
amino acid, hypusine [N′-(4-amino-2-hydroxybutyl) lysine]. This unusual amino acid is
formed post-translationally by modification of a single specific lysine residue in an eIF-4D
precursor protein. Two separate eIF-4D precursors, each of which contains a lysine residue
in place of the hypusine residue and each of which thereby serves as a protein substrate for
the hypusine modification, were purified from DL-2-difluoromethylornithine-treated Chinese …