Identification of an antigen-specific B cell population
J Newman, JS Rice, C Wang, SL Harris… - Journal of immunological …, 2003 - Elsevier
The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire
has been a formidable obstacle to understanding both protective and pathogenic antibody
responses. We have developed a tetramer-based technique for identifying antigen-specific
B cells. Biotin-labeled antigen is made tetrameric by interaction with streptavidin. The
enhanced avidity of this antigenic compound for the B cell membrane permits the
visualization, characterization and isolation of antigen-specific B cells.
has been a formidable obstacle to understanding both protective and pathogenic antibody
responses. We have developed a tetramer-based technique for identifying antigen-specific
B cells. Biotin-labeled antigen is made tetrameric by interaction with streptavidin. The
enhanced avidity of this antigenic compound for the B cell membrane permits the
visualization, characterization and isolation of antigen-specific B cells.
