[HTML][HTML] The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers
L Thomassin, CC Werneck, TJ Broekelmann… - Journal of Biological …, 2005 - ASBMB
These studies were undertaken to determine how lysyl oxidase (LOX) and lysyl oxidase like-
1 (LOXL) enzymes are targeted to their substrates in the extracellular matrix. Full-length
LOX/LOXL and constructs containing just the pro-regions of each enzyme localized to elastic
fibers when expressed in cultured cells. However, the LOXL catalytic domain without the pro-
region was secreted into the medium but did not associate with matrix. Ligand blot and
mammalian two-hybrid assays confirmed an interaction between tropoelastin and the pro …
1 (LOXL) enzymes are targeted to their substrates in the extracellular matrix. Full-length
LOX/LOXL and constructs containing just the pro-regions of each enzyme localized to elastic
fibers when expressed in cultured cells. However, the LOXL catalytic domain without the pro-
region was secreted into the medium but did not associate with matrix. Ligand blot and
mammalian two-hybrid assays confirmed an interaction between tropoelastin and the pro …