The binding of fatty acids to cytoplasmic proteins: binding to Z protein in liver and other tissues of the rat
S Mishkin, L Stein, Z Gatmaitan, IM Arias - Biochemical and biophysical …, 1972 - Elsevier
S Mishkin, L Stein, Z Gatmaitan, IM Arias
Biochemical and biophysical research communications, 1972•ElsevierA cytoplasmic binding protein (Z protein) has now been shown to have a high affinity for fatty
acids. This protein is present in the cytoplasm of rat liver, myocardium, skeletal muscle,
intestinal mucosa, adipose tissue and kidney. Oleic acid was reversibly bound to Z with a
Km of 2.8× 10− 6 M; flavi-spidic acid competitively inhibited this process. Relative binding of
fatty acids to Z increased with chain length and decreased with unsaturation. Triolein,
cholesterol and several bile salts were not bound by Z protein.
acids. This protein is present in the cytoplasm of rat liver, myocardium, skeletal muscle,
intestinal mucosa, adipose tissue and kidney. Oleic acid was reversibly bound to Z with a
Km of 2.8× 10− 6 M; flavi-spidic acid competitively inhibited this process. Relative binding of
fatty acids to Z increased with chain length and decreased with unsaturation. Triolein,
cholesterol and several bile salts were not bound by Z protein.
Abstract
A cytoplasmic binding protein (Z protein) has now been shown to have a high affinity for fatty acids. This protein is present in the cytoplasm of rat liver, myocardium, skeletal muscle, intestinal mucosa, adipose tissue and kidney. Oleic acid was reversibly bound to Z with a Km of 2.8 × 10−6M; flavi-spidic acid competitively inhibited this process. Relative binding of fatty acids to Z increased with chain length and decreased with unsaturation. Triolein, cholesterol and several bile salts were not bound by Z protein.
Elsevier