Lipofibroblasts in structurally normal, fibrotic, and emphysematous human lungs
J Schipke, S Kuhlmann, J Hegermann… - American Journal of …, 2021 - atsjournals.org
American Journal of Respiratory and Critical Care Medicine, 2021•atsjournals.org
Methods The material investigated in this study was taken from explanted fibrotic (n= 10)
and emphysematous (n= 10) human lungs as well as from the periphery of tumor resections
(n= 7); it was then directly fixed in 4% buffered formaldehyde and embedded in paraffin or in
1.5% glutaraldehyde and 1.5% paraformaldehyde in a 0.15 M HEPES buffer. The study was
designed and performed following the requirements of the local ethics committee at
Hannover Medical School (ethics vote number 2702-2015). Paraffin sections were stained …
and emphysematous (n= 10) human lungs as well as from the periphery of tumor resections
(n= 7); it was then directly fixed in 4% buffered formaldehyde and embedded in paraffin or in
1.5% glutaraldehyde and 1.5% paraformaldehyde in a 0.15 M HEPES buffer. The study was
designed and performed following the requirements of the local ethics committee at
Hannover Medical School (ethics vote number 2702-2015). Paraffin sections were stained …
Methods
The material investigated in this study was taken from explanted fibrotic (n= 10) and emphysematous (n= 10) human lungs as well as from the periphery of tumor resections (n= 7); it was then directly fixed in 4% buffered formaldehyde and embedded in paraffin or in 1.5% glutaraldehyde and 1.5% paraformaldehyde in a 0.15 M HEPES buffer. The study was designed and performed following the requirements of the local ethics committee at Hannover Medical School (ethics vote number 2702-2015). Paraffin sections were stained for ADFP (adipose differentiation-related protein; marker for lipofibroblasts) and WGA (wheat germ agglutinin) to identify the septal localization of ADFP+ cells. Paraffin sections were also stained for ADFP, 4′, 6-diamidino-2-phenylindol (DAPI; marker for nuclei), and other cell markers, such as vimentin (cells of mesenchymal origin), pro–SP-C (pro–surfactant protein C; marker for alveolar epithelial type 2 cells), ACTA2 (actin α2; marker for myofibroblasts), and CD68 (cluster of differentiation 68; marker for macrophages).
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