[HTML][HTML] Protocol for the generation of murine bronchiolospheres
AI Vazquez-Armendariz, W Seeger, S Herold… - STAR protocols, 2021 - Elsevier
Organoid models have been shown to be valuable tools for studying epithelial-
mesenchymal crosstalk during biological and pathological settings. Our data identified
ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the
conducting airway niche. Here, we provide a detailed protocol for culturing airway
organoids, or bronchiolospheres, which provide an assessment of the ability of
mesenchymal cells to support club-cell growth. For complete details on the use and …
mesenchymal crosstalk during biological and pathological settings. Our data identified
ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the
conducting airway niche. Here, we provide a detailed protocol for culturing airway
organoids, or bronchiolospheres, which provide an assessment of the ability of
mesenchymal cells to support club-cell growth. For complete details on the use and …
Summary
Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth.
For complete details on the use and execution of this protocol, please refer to Moiseenko et al. (2020).
Elsevier