Generation of multi-cellular human liver organoids from pluripotent stem cells
WL Thompson, T Takebe - Methods in cell biology, 2020 - Elsevier
A growing number of in vitro hepatic models exist to study human genetics, liver biology,
disease modeling and drug development and range from 2D hepatocytes to 3D multi-
cellular tissues that are derived from human stem cells. However, stem cell-based models
generally suffer from batch-, clone-and donor-dependent variability, hindering broader
usage in diverse biomedical applications. To circumvent this challenge, we herein describe
a reproducible protocol to generate human liver organoids in 20–25 days derived from …
disease modeling and drug development and range from 2D hepatocytes to 3D multi-
cellular tissues that are derived from human stem cells. However, stem cell-based models
generally suffer from batch-, clone-and donor-dependent variability, hindering broader
usage in diverse biomedical applications. To circumvent this challenge, we herein describe
a reproducible protocol to generate human liver organoids in 20–25 days derived from …